- 10COMPATIBLE WITH RFC
- 12COMPATIBLE WITH RFC
- 21Illegal BamHI site found at 507
- 23COMPATIBLE WITH RFC
- 25COMPATIBLE WITH RFC
- 1000COMPATIBLE WITH RFC
We also had other ways of localizing the transcription factor to the plasma membrane e.g. with a C-terminal CAAX box. In the end this design fitted better to our cloning flow since it reuses some of the protein domains also used elsewhere in our project.
S. cerevisiae, E. coli, herpes simplex virus.