Coding
Part:BBa_K3617002:Design
Designed by: David Nørgaard Essenbæk Group: iGEM20_UCopenhagen (2020-10-22)
Interleukin-10 receptor type 1 - C-ubiquitin-LexA-VP16
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 628
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1269
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We chose to leave in both extracellular domains, since both looked to be part of interleukin binding.
Source
Fusion protein consisting of domains from H. sapiens, S. cerivisiae, e.coli, herpes simplex virus.