Part:BBa_K3612001:Design
Lolium perenne antifreeze protein (LpAFP)
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 70
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Sequence optimized for E. coli.
Source
GenBank accession number: AJ277399.1
References
(1) Xiang H, Yang X, Ke L, Hu Y. The properties, biotechnologies, and applications of antifreeze proteins. Int J Biol Macromol. 2020 Jun 15;153:661–75.
(2) Mahatabuddin S, Tsuda S. Applications of antifreeze proteins: Practical use of the quality products from Japanese fishes. In: Advances in Experimental Medicine and Biology. Springer New York LLC; 2018. p. 321–37.
(3) Balamurugan S, Ann JS, Varghese IP, Murugan SB, Harish MC, Kumar SR, et al. Heterologous expression of Lolium perenne antifreeze protein confers chilling tolerance in tomato. J Integr Agric. 2018 May 1;17(5):1128–36.
(4) Bredow, M., Vanderbeld, B., & Walker, V. K. (2017). Ice-binding proteins confer freezing tolerance in transgenic Arabidopsis thaliana. Plant Biotechnology Journal, 15(1), 68–81.
(5) Bredow M, Walker VK. Ice-binding proteins in plants. Front Plant Sci. 2017;8(December):1–15.
(6) Davies, P. L. (2014). Ice-binding proteins: A remarkable diversity of structures for stopping and starting ice growth. Trends in Biochemical Sciences, Vol. 39, pp. 548–555.
(7) Middleton, A. J., Marshall, C. B., Faucher, F., Bar-Dolev, M., Braslavsky, I., Campbell, R. L., … Davies, P. L. (2012). Antifreeze protein from freeze-tolerant grass has a beta-roll fold with an irregularly structured ice-binding site. Journal of Molecular Biology, 416(5), 713–724.
(8) Lauersen, K. J., Brown, A., Middleton, A., Davies, P. L., & Walker, V. K. (2011). Expression and characterization of an antifreeze protein from the perennial rye grass, Lolium perenne. Cryobiology.