Coding

Part:BBa_K3611010

Designed by: Peixuan Zheng   Group: iGEM20_NEU_CHINA   (2020-10-26)

The coding gene of ACE2-PmrB recombinant protein.


This is the coding gene of egfp fusing AAV protein degradation tag. To reduce the sensor basal background, we use protease-based post-transcriptional degradation regulation. By adding a protein degradation tag (AAV) to the reporter protein EGFP, the output basal expression was reduced, also the sensor’s output level.

T--NEU_CHINA--np47.png

Fig. 1 Characterization of AAV protein degradation tag. The cells are induced by 5 varying concentrates of IPTG (0, 10-6, 10-5, 10-4, 10-3 mmol/L) after 1h.



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


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Categories
Parameters
None