Part:BBa_K3610058:Design
BAK1 ectodomain / LargeBit for C. reinhardtii
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 93
Illegal NheI site found at 925 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 855
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
We needed localization at the membrane, thus we needed a signal peptide native to C. reinhardtii.
The self-cleaving peptide from the human foot-and-mouth-disease virus allows expression of multiple protein coding fragments with the same promoter.
Source
BBa_K2136017, BBa_K3610012, BBa_K3610004, BBa_K1150016, BBa_K416001, BBa_K3610015
References
Barahimipour, Rouhollah; Strenkert, Daniela; Neupert, Juliane; Schroda, Michael; Merchant, Sabeeha S.; Bock, Ralph (2015): Dissecting the contributions of GC content and codon usage to gene expression in the model alga Chlamydomonas reinhardtii. In: Plant J 84 (4), S. 704–717. DOI: 10.1111/tpj.13033.
Chinchilla, Delphine; Shan, Libo; He, Ping; Vries, Sacco de; Kemmerling, Birgit (2009): One for all: the receptor-associated kinase BAK1. In: Trends Plant Sci 14 (10), S. 535–541. DOI: 10.1016/j.tplants.2009.08.002.
England, Christopher G.; Ehlerding, Emily B.; Cai, Weibo (2016): NanoLuc: A Small Luciferase Is Brightening Up the Field of Bioluminescence. In: Bioconjug Chem 27 (5), S. 1175–1187. DOI: 10.1021/acs.bioconjchem.6b00112.
Koller, Teresa; Bent, Andrew F. (2014): FLS2-BAK1 extracellular domain interaction sites required for defense signaling activation. In: PLOS ONE 9 (10), e111185-e111185. DOI: 10.1371/journal.pone.0111185.
Ranf, Stefanie (2018): Pattern Recognition Receptors—Versatile Genetic Tools for Engineering Broad-Spectrum Disease Resistance in Crops. In: Agronomy 8, S. 134. DOI: 10.3390/agronomy8080134.