Composite

Part:BBa_K3610057

Designed by: Jonas Sebastian Trottmann   Group: iGEM20_UZurich   (2020-10-09)


FRB / SmallBit NanoLuc for S. cerevisiae

This part encodes the SmallBit protein from the split-NanoLuc system fused to the FKBP–rapamycin binding (FRB) domain of the mammalian target of rapamycin (mTOR). This part can be used together with the Part:BBa_K3610055 to test the dimerization of the codon optimized split-NanoLuc proteins via rapamycin induced dimerization of FKBP and FRB.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None