Composite

Part:BBa_K3606915

Designed by: Yuchang Lee   Group: iGEM20_Fudan   (2020-10-27)


P2 driven mcbABCD; PtetR driven mcbEFG; luxPR driven tetR

Usage and Biology:

This part completely integrates our quorum sensing system. LuxPR driven tetR is our "switch" for quorum sensing, and P2 driven mcbABCD and PtetR driven mcbEFG are our MccB17 synthesis and secretion system to respond to luxPR driven tetR.

design:

In this part, the expression of tetR is driven by LuxpR; tetR will block ptetR, resulting in the inability to express McbEFG; and P2 as a constitutive promoter will continue to express McbABCD.


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 5276
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 5233
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 5276
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 5276
    Illegal PstI site found at 2297
    Illegal PstI site found at 2330
    Illegal NgoMIV site found at 1959
    Illegal AgeI site found at 2131
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None