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Coding
Part:BBa_K3606810
Designed by: Mingwei Li, Gaochen Zhang Group: iGEM20_Fudan (2020-10-24)
mcbE
Usage and Biology:
McbE works as channel protein responsible for efflux of MccB17.
Design:
Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbE into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbE expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbE product was produced.
Results:
We have successfully expressed mcbE for the fundamental validation of the antimicrobial peptide(mccb17) expression.
Figure1. gel map for mcbA, mcbC, mcbE and mcbF
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |