Coding

Part:BBa_K3606807

Designed by: Yuanyuan Liu   Group: iGEM20_Fudan   (2020-10-24)


mcbB

Usage and Biology:

McbB works as synthase responsible for the maturation of MccB17.

Design:

Since we encountered difficulties in the expression of McbABCDEFG at the beginning, we decided to check whether the McbA/B/C/D/E/F/G genes can be expressed and translated separately. We successfully cloned McbB into the pGEX plasmid and transcribed it into BL21. After 5 hours of induction of McbB expression with IPTG, the whole protein SDS-PAGE was used to detect whether the McbB product was produced.

Results:

More information：https://parts.igem.org/Part:BBa_K3606814

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None