Composite

Part:BBa_K3606094

Designed by: Yuanyuan Liu   Group: iGEM20_Fudan   (2020-10-23)


PlacIq_lacI_Ptac driven mcbD

This is a plamid with high expression level，McbD works as synthase responsible for the maturation of MccB17.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 403
    Illegal PstI site found at 436
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 403
    Illegal PstI site found at 436
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 310
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 403
    Illegal PstI site found at 436
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 403
    Illegal PstI site found at 436
    Illegal NgoMIV site found at 65
    Illegal AgeI site found at 237
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None