Composite

Part:BBa_K3606081

Designed by: Yuanyuan Liu   Group: iGEM20_Fudan   (2020-10-23)


PlacIq_lacI_Ptac driven NSP4-CaAP

PlacIq_lacI_Ptac driven NSP4-CaAP is a part of our signal-peptide guided CaAP secretion system. <p>To test the secretion efficiency of our 5 secretion peptides fused CaAP(Calcium Absorption Peptide) guided by 5 secretion peptides, we put them under the regulation of the iPTG inducible promoter, Ptac, and expressed them in E.coli. BL21 strain respectively. Thus, quantified characterization of these secretion peptides fused with CaAP is expected to be achieved through the different quantity of the target protein, namely CaAP, in the bacterial lysate and the culture medium.

The 5 signal peptides candidates for CaAP secretion are listed below.

Signal Peptide Number for Registry
NSP4 BBa_K3606042
OmpA BBa_K3606043
DsbA BBa_K3606030
PelB BBa_K208004
PhoA BBa_K808028

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1233
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1324
    Illegal AgeI site found at 1411
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None