Composite

Part:BBa_K3606080

Designed by: Yuanyuan Liu   Group: iGEM20_Fudan   (2020-10-23)


PlacIq_lacI_Ptac driven CaAP

The specially designed CaAP(Calcium Absorption Peptide) can promote calcium absorption. We put the expression of it under the regulation of Ptac, an IPTG inducible promoter, to achieve the better qualification of our secretion peptides. Thus, the expression intensity of the gene of interest can be quantified and regulated.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 107
  • 1000
    COMPATIBLE WITH RFC[1000]

References:

[1] Mutalik VK, Guimaraes JC, Cambray G, Lam C, Christoffersen MJ, Mai QA, Tran AB, Paull M, Keasling JD, Arkin AP, Endy D. Precise and reliable gene expression via standard transcription and translation initiation elements. Nat Methods. 2013 Apr;10(4):354-60. doi: 10.1038/nmeth.2404. Epub 2013 Mar 10. PMID: 23474465.


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