Composite

Part:BBa_K3606044:Design

Designed by: Zhenru Zhang   Group: iGEM20_Fudan   (2020-10-23)


luxPR driven CaAP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 26
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 142
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The expression of CaAP(Calcium Absorption Peptide) is regulated by luxpR(HS100) promoter so that the engineered probiotics only express

and secrete CaAP when their population reaches a certain level.


Source

The sequences of subparts are obtained from E.coli. genome.

References