![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3606037:Design
Designed by: Zhenru Zhang Group: iGEM20_Fudan (2020-10-23)
PlacIq_lacI_Ptac driven Nsp4-MBP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1233
Illegal BglII site found at 1729 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1357
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1427
Design Notes
We linked NSP4 to the N terminal of MBP for the test and verification of secretion led by NSP4.
Source
The sequences of subparts are obtained from E.coli. genome.