Composite

Part:BBa_K3606037:Design

Designed by: Zhenru Zhang   Group: iGEM20_Fudan   (2020-10-23)


PlacIq_lacI_Ptac driven Nsp4-MBP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1233
    Illegal BglII site found at 1729
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1427


Design Notes

We linked NSP4 to the N terminal of MBP for the test and verification of secretion led by NSP4.


Source

The sequences of subparts are obtained from E.coli. genome.

References