![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3606032:Design
Designed by: Zhenru Zhang Group: iGEM20_Fudan (2020-10-23)
OmpA-CaAP
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 138
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
The composite part OmpA_CaAP is a combination of signal peptide OmpA and CaAP(Calcium Absorption Peptide). The sequence of OmpA is obtained from literature with further codon optimization. Then we fused OmpA (BBa_K3606030) to the N-terminal of CaAP(BBa_K3606000) for its secretion into the extracellular space.
Source
The sequences of subparts are obtained from E.coli. genome and have gone through codon optimization.