Part:BBa_K3605006
β-glucosidase from Gluconacetobacter xylinus.
β-glucosidase is encoded by bglxa gene in Gluconacetobacter xylinus. β-glucosidase and endoglucanase (CMCax) both can hydrolyze tangled glucan chains when there is a failure chain arrangement and are both crucial for bacterial cellulose synthesis.
This part is β-glucosidase encoded by bglxa gene with 2202bp in Gluconacetobacter xylinus. Both of the β-glucosidase and endoglucanase (CMCax, BBa_K3605004) can hydrolyze tangled glucan chains when there is a failure chain arrangement and are both crucial for bacterial cellulose synthesis. This gene was also amplified using PCR method, and then inserted into the vector pSB1C3. The identification result is showed in Fig.1.
Fig.1. The result of β-glucosidase gene cloning. M: Marker; 1: PCR result of β-glucosidase; 2: Digestion of pSB1C3 containing β-glucosidase.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 550
Illegal NgoMIV site found at 1602 - 1000COMPATIBLE WITH RFC[1000]
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