Part:BBa_K3605003

Bacteria cellulose synthase (Bcs) from Gluconacetobacter xylinus.

This part is bacterial cellulose synthase (Bcs) complex (9102bp) from Gluconacetobacter xylinus. It contains 4 subunits BcsA, BcsB, BcsC and BcsD, which catalyzes the bacterial cellulose synthesis, using uridine-5’-phosphate-α-D-glucose (UDPG). This is the last reaction for Bacterial cellulose production from glucose. The reaction catalyzed by this enzyme is as follows:

Due to the long sequence (9102bp), this enzyme complex was synthesized directly and connected into pUC57 vector firstly, then it was inserted into the plasmid pSB1C3. The identification result is showed in Fig.1.

Fig.1. The gene cloning result of Bcs complex. M: Marker; 1: Digestion of pUC57 vector containing Bcs complex; 2: Digestion of pSB1C3 vector containing Bsc complex.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 740
  Illegal BglII site found at 1136
  Illegal XhoI site found at 232
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 3387
  Illegal NgoMIV site found at 4617
  Illegal NgoMIV site found at 4687
  Illegal NgoMIV site found at 5569
  Illegal NgoMIV site found at 5758
  Illegal NgoMIV site found at 8925
  Illegal NgoMIV site found at 8929
  Illegal AgeI site found at 7281
  Illegal AgeI site found at 7558
  Illegal AgeI site found at 7818
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 6141
  Illegal BsaI.rc site found at 982
  Illegal BsaI.rc site found at 1705