Coding

Part:BBa_K3605002:Design

Designed by: Guangzhong Zhang   Group: iGEM20_JNFLS   (2020-10-21)


Phosphoglucomutase (PGM) and UDP-glucose pyrophosphorylase (UGPase) from Gluconacetobacter xylinus.


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 184
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 757


Design Notes

To avoid the recognition sequence of some restriction enzymes, some bases are modified.


Source

it comes from the genomic sequence of Gluconacetobacter xylinus.

References