RNA

Part:BBa_K3602004:Design

Designed by: Flora Fuglsang   Group: iGEM20_SDU-Denmark   (2020-10-23)


rs6983267-G sgRNA Cas13a


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The scaffold sequence was designed to match the requirements for cas13a according to Gootenberg et al. 2017. (https://science.sciencemag.org/content/356/6336/438)

The T7 promoter was chosen from neb.com for high yield RNA synthesis. (https://international.neb.com/protocols/2015/11/24/sgrna-synthesis-using-the-hiscribe-quick-t7-high-yield-rna-synthesis-kit-neb-e2050)


Source

The rs6983267 is taken from SNPedia (https://www.snpedia.com/index.php/SNPedia?fbclid=IwAR16nut2oLDQcZcoL3nrIiaVT9QPNiCDs_tF04crjVm1f4PKeTHe3IzWYLg)

References