Coding

Part:BBa_K3602001:Design

Designed by: Flora Fuglsang   Group: iGEM20_SDU-Denmark   (2020-10-23)


Cas13a w. tags, E. coli K12 optimized


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2978
    Illegal BglII site found at 3086
    Illegal BglII site found at 3573
    Illegal BamHI site found at 244
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1663
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 131
    Illegal BsaI.rc site found at 2815


Design Notes

The protein is designed from Gootenberg et al. 2017 [1]. When designed the sequence was codon-optimized for E. coli K12 and it was ensured that the iGEM restriction sites were not present in the sequence.

Source

This sequence is matching the one from Gootenberg et al. 2017, which originates from LwC2c2 [1].

References

[1] https://science.sciencemag.org/content/356/6336/438