Plasmid

Part:BBa_K3600016:Design

Designed by: yasmine koubaa   Group: iGEM20_EPFL   (2020-10-27)


Lys2-GSH1pr-mScarleti cassette


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 994
    Illegal EcoRI site found at 1239
    Illegal EcoRI site found at 1819
    Illegal XbaI site found at 939
    Illegal XbaI site found at 1248
    Illegal XbaI site found at 1996
    Illegal SpeI site found at 2835
    Illegal PstI site found at 2844
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 994
    Illegal EcoRI site found at 1239
    Illegal EcoRI site found at 1819
    Illegal SpeI site found at 2835
    Illegal PstI site found at 2844
    Illegal NotI site found at 723
    Illegal NotI site found at 3399
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal suffix found in sequence at 2566
    Illegal EcoRI site found at 994
    Illegal EcoRI site found at 1239
    Illegal EcoRI site found at 1819
    Illegal BglII site found at 2437
    Illegal BglII site found at 2859
    Illegal BamHI site found at 2871
    Illegal XhoI site found at 1684
    Illegal XhoI site found at 2880
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 994
    Illegal EcoRI site found at 1239
    Illegal EcoRI site found at 1819
    Illegal XbaI site found at 939
    Illegal XbaI site found at 1248
    Illegal XbaI site found at 1996
    Illegal SpeI site found at 2835
    Illegal PstI site found at 2844
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 994
    Illegal EcoRI site found at 1239
    Illegal EcoRI site found at 1819
    Illegal XbaI site found at 939
    Illegal XbaI site found at 1248
    Illegal XbaI site found at 1996
    Illegal SpeI site found at 2835
    Illegal PstI site found at 2844
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1681
    Illegal SapI.rc site found at 756


Design Notes

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Source

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References