Coding

Part:BBa_K3599035

Designed by: Zijia Liu   Group: iGEM20_TPR_China   (2020-10-24)


baPAD

an enzyme which was designed into the artificial biosynthesis pathway of 4VA synthesis. The original function of baPAD was converting 4-coumaric acid into 4-hydroxystyrene. The codon usage was optimized for expression in E. coli.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 220
  • 1000
    COMPATIBLE WITH RFC[1000]


Reference

Kang, S. Y., Choi, O., Lee, J. K., Ahn, J. O., Ahn, J. S., Hwang, B. Y., & Hong, Y. S. (2015). Artificial de novo biosynthesis of hydroxystyrene derivatives in a tyrosine overproducing Escherichia coli strain. Microbial cell factories, 14(1), 1-11. 10.1186/s12934-015-0268-7

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