Composite

Part:BBa_K3596056:Design

Designed by: Xinyu Liu   Group: iGEM20_GreatBay_SZ   (2020-10-23)


BIOT with RBS mut4 and Pilin gene mut4A


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 2393
    Illegal BglII site found at 2857
    Illegal BglII site found at 4658
    Illegal BamHI site found at 1073
    Illegal BamHI site found at 3182
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1300
    Illegal AgeI site found at 1146
    Illegal AgeI site found at 1439
    Illegal AgeI site found at 2002
    Illegal AgeI site found at 4626
    Illegal AgeI site found at 6914
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 7920
    Illegal SapI.rc site found at 4262


Design Notes

The RBS was designed with RBS Calculator and the pilin protein was R41E, K44D, S49D, Q55E mutated form the wildtype.


Source

E. coli O157:H7, G. sulfurreducens

References