Part:BBa_K3596051
BIOT RBS mut4
BIOT is a basic design for the expression of Geobacter sulfurreducens' pilin-based electrically conductive protein nanowires (e-PNs)[1]. The system includes a tac promoter which promotes the pilin gene(pilA) to express protein monomers; the monomers are later assembled into protein nanowires by the TypeIV pilin assembly system, which is constructed from the gene cluster at the downstream of the pilin gene(figure 1). The e-PNs can generate electric potential with the presence of water molecules in the air, which can be further modified for other purposes.
Figure 1: A Construction of BIOT gene circuit
In order to improve the protein nanowire, we mutated the RBSs of the TypeIV pilin assembly system. This part codes for BIOT with the hofB RBS and ppdA RBS replaced by hofB RBS mut2 and ppdA RBS mut1 respectively .The e-PN productivity of this part is shown in figure 2.
Figure 2: Different pilin productivity of engineered BIOT system with different RBS.
References
[1] Liu X, Gao H, Ward J E, et al. Power generation from ambient humidity using protein nanowires[J]. Nature, 2020, 578(7796): 550-554.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 2393
Illegal BglII site found at 2857
Illegal BglII site found at 4658
Illegal BamHI site found at 1073
Illegal BamHI site found at 3182 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1300
Illegal AgeI site found at 1146
Illegal AgeI site found at 1439
Illegal AgeI site found at 2002
Illegal AgeI site found at 4626
Illegal AgeI site found at 6914 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 7920
Illegal SapI.rc site found at 4262
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