Composite

Part:BBa_K3594004:Design

Designed by: Runhan Luo   Group: iGEM20_SHSBNU_China   (2020-10-23)


Guaiacol Degrading Enzyme System


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1332
    Illegal XhoI site found at 394
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 34
    Illegal NgoMIV site found at 355
    Illegal NgoMIV site found at 406
    Illegal NgoMIV site found at 841
    Illegal NgoMIV site found at 1000
    Illegal NgoMIV site found at 1180
    Illegal NgoMIV site found at 1305
    Illegal AgeI site found at 1416
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

It functions in the presence of IPTG


Source

https://amb-express.springeropen.com/articles/10.1186/s13568-019-0759-8#Tab1

References