Coding

Part:BBa_K3593011:Design

Designed by: Wanning He   Group: iGEM20_GreatBay_SCIE   (2020-10-08)


Single-Chain Fragment Variable (anti α-amatinin)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 98
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

you may add antigen tag that is more specific than 6*His tag. you may mutate this gene to reach a higher specificity.

Source

Artificially synthesized

References

1. Zhang X, He K, Zhao R, Feng T, Wei D. Development of a Single Chain Variable Fragment Antibody and Application as Amatoxin Recognition Molecule in Surface Plasmon Resonance Sensors. Food Anal Methods. 2016. doi:10.1007/s12161-016-0509-3

2. SAbPred: ABodyBuilder http://opig.stats.ox.ac.uk/webapps/newsabdab/sabpred/abodybuilder/