Part:BBa_K3593010
ssDNA, capture probe of control line in method without RPA
This ssDNA can be immobilized on the control line region of test strip. It is complementary with the best 1 aptamer conjugated to a reporter Au nanoparticle, thus reporting a red color to verify the function of lateral flow construction
Sequence and features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Characterisation of this part
Design
Besides aptamers, we have some short single stranded DNA known as 'probes', which binds to other single stranded DNA. BBa_K3593010 is one of those so-called 'probes' in our design, and play important roles in our hardware, in which it binds and immobilizes the aptamer. Its 5' end is conjugated to biotin[1] to bind with streptavidin.
References
1.Jauset-Rubio M, Svobodová M, Mairal T, et al. Aptamer Lateral Flow Assays for Ultrasensitive Detection of β-Conglutin Combining Recombinase Polymerase Amplification and Tailed Primers. Anal Chem. 2016;88(21):10701-10709. doi:10.1021/acs.analchem.6b03256
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