Intermediate

Part:BBa_K359006:Design

Designed by: Cveta Manassieva   Group: iGEM10_Waterloo   (2010-09-02)


AIP sensor and generator


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 2115
    Illegal NheI site found at 2138
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 426
    Illegal BamHI site found at 1310
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Used J23107, a mild constitutive promoter, to avoid inserting too many unnatural proteins into the E.coli membrane.


Source

These parts were designed and constructed by the Cambridge 2007 iGEM Team: The original sequences for agrC and agrA were taken from the S. aureus Sequencing Project (results for strain NCTC8325) at Oklahoma University (http://www.genome.ou.edu/staph.html). The sequences were then codon-optimised for E. coli using GeneDesigner from DNA2.0 (http://www.dna20.com), and then synthesised by that company.

References