Composite
Part:BBa_K3590037:Design
Designed by: Barbora Hrnč�řov� Group: iGEM20_Brno_Czech_Republic (2020-10-18)
Scaffoldin for lysis of cyanobacteria
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1476
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1476
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1476
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1476
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 223
Illegal SapI.rc site found at 1538
Design Notes
For cloning purposes, attaaGAATTC (containing EcoRI) was added to 5' end and tttAAGCTT (containing HindIII) was added to 3' end. On both ends of the sequence, there are several nucleotides that help the restriction enzymes to interact with the sequence.
Source
This is a synthetic part. The sources of each fragment can be found at the individual page of each Basic Part that is present in this Composite Part.