Coding

Part:BBa_K3590008:Design

Designed by: Barbora Hrnčířová   Group: iGEM20_Brno_Czech_Republic   (2020-09-01)


SacB_v4


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 61
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The BioBrick BBa_K2273063 designed by Henri Deda from iGEM17_TU_Dresden was codon-optimized for higher translation efficiency in Bacillus subtilis and for easier synthesis. There are four BioBricks coding the SacB in total - BBa_K3590005, BBa_K3590006, BBa_K3590007.


Source

BioBrick BBa_K2273063 designed by Henri Deda from iGEM17_TU_Dresden.

References