Composite

Part:BBa_K3589206:Design

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-25)


Level 1 – Wildtype marLac + cCA + HA-RGS-8His


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1379
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1379
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1379
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1379
    Illegal NgoMIV site found at 1239
    Illegal NgoMIV site found at 1958
    Illegal NgoMIV site found at 2409
    Illegal NgoMIV site found at 2929
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is compatible with the MoClo standard and codon optimized for C. reinhardtii.

Source

This part was created using the MoClo system.

References