Composite

Part:BBa_K3589205:Design

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-25)


Level 1 – Mutant BaLac + cCA + HA-RGS-8His


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1617
    Illegal BglII site found at 2576
    Illegal BamHI site found at 1956
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1170
    Illegal PstI site found at 1257
    Illegal PstI site found at 1321
    Illegal PstI site found at 1693
    Illegal PstI site found at 1981
    Illegal PstI site found at 2058
    Illegal NgoMIV site found at 3690
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is compatible with the MoClo standard and is codon optimized for C. reinhardtii.

Source

This part was created using the MoClo system.

References