Composite

Part:BBa_K3589201:Design

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-23)


Level 1 – Mutant BaLac + HA (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 1103
    Illegal PstI site found at 1190
    Illegal PstI site found at 1254
    Illegal PstI site found at 1626
    Illegal PstI site found at 1914
    Illegal PstI site found at 1991
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 249
    Illegal PstI site found at 1103
    Illegal PstI site found at 1190
    Illegal PstI site found at 1254
    Illegal PstI site found at 1626
    Illegal PstI site found at 1914
    Illegal PstI site found at 1991
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1550
    Illegal BglII site found at 2509
    Illegal BamHI site found at 1889
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 1103
    Illegal PstI site found at 1190
    Illegal PstI site found at 1254
    Illegal PstI site found at 1626
    Illegal PstI site found at 1914
    Illegal PstI site found at 1991
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 1103
    Illegal PstI site found at 1190
    Illegal PstI site found at 1254
    Illegal PstI site found at 1626
    Illegal PstI site found at 1914
    Illegal PstI site found at 1991
    Illegal NgoMIV site found at 3630
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This part is MoClo compatible and codon optimized for C. reinhardtii.

Source

This part was created using the MoClo standard.

References