Intermediate

Part:BBa_K3589108:Design

Designed by: Yannik Schermer   Group: iGEM20_TU_Kaiserslautern   (2020-10-23)


Mutant laccase from Botrytis aclada for Chlamydomonas reinhardtii (Phytobrick)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
    Illegal PstI site found at 1496
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
    Illegal PstI site found at 1496
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1055
    Illegal BglII site found at 2014
    Illegal BamHI site found at 1394
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
    Illegal PstI site found at 1496
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 608
    Illegal PstI site found at 695
    Illegal PstI site found at 759
    Illegal PstI site found at 1131
    Illegal PstI site found at 1419
    Illegal PstI site found at 1496
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

he natural secretion signal of Botrytis aclada was removed. This part is codon-optimized for Chlamydomonas reinhardtii. Additionally, it is compatible with Phytobricks /MoClo parts.

Source

This part originates from Botrytis aclada (Scheiblbrandner, S., 2017) and is condon-optimized for Chlamydomonas reinhardtii.

References