Composite

Part:BBa_K3585007

Designed by: Kong Yangyang   Group: iGEM20_Shanghai_HS_United   (2020-10-14)


pJ23100-butyrate-J23200-lacY

In E. coli, the presence of glucose inhibits the synthesis of many catabolic enzymes, a phenomenon called "glucose effect" or "glucose inhibition". Studies have shown that overexpression of LacY can eliminate the glucose effect. We use the strong promoter J23200 to continuously overexpress LacY protein to achieve simultaneous metabolism of glucose and galactose. Continuous expression of butyrate synthesis pathway enzymes through a constitutive promoter, so the strain can degrade galactose and synthesize butyrate. Combining the butyrate synthesis pathway enzyme and LacY protein, the engineered strain can effectively degrade galactose in the presence of glucose.

T--Shanghai HS United-BBa K3585007 fig 00.png

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 1240
    Illegal NheI site found at 8195
    Illegal NheI site found at 8218
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 6272
    Illegal BglII site found at 7408
    Illegal BglII site found at 7549
    Illegal XhoI site found at 1245
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 9008
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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