Plasmid
Part:BBa_K3582500:Design
Designed by: Avadhoot Jadhav Group: iGEM20_IISER-Pune-India (2020-10-24)
Modified Pet28a(+) expression vector
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 105
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 137
Illegal NgoMIV site found at 1725
Illegal NgoMIV site found at 1885
Illegal NgoMIV site found at 4932 - 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 2805
Design Notes
Removed excess tags and non required regions. RFC 10 criteria satisfied.
Source
Snapgene