Coding
Part:BBa_K3582301:Design
Designed by: Jatin Bedi, Avadhoot Jadhav Group: iGEM20_IISER-Pune-India (2020-10-22)
Basigin protein
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 67
Illegal NgoMIV site found at 730 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 358
Illegal SapI.rc site found at 330
Illegal SapI.rc site found at 747
Design Notes
- 1] RFC10 criteria satisfied
- 2]Strep Ⅱ tag gene added for purification
- 3] Codon optimized with E-coli K-12 strain
Source
Reverse Translation.