Coding
Part:BBa_K3582206:Design
Designed by: Avadhoot Jadhav, Gunwant Patil Group: iGEM20_IISER-Pune-India (2020-10-22)
Inhibitory Sequence 2 for EPCR-PfEMP1 Grafted In Cyclotide
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 112
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 143
Illegal BsaI.rc site found at 109
Design Notes
- 1] RFC10 criteria satisfied
- 2] Strep II tag inserted in loop 3
- 3] Codon optimized with E-coli K-12 strain.
- 4] Inhibitor 1 grafted in loop 6
- 5] C and N inteins added for circularization
Source
Reverse Translation