Part:BBa_K3582102

CIDRa domain from IT4var07 PfEMP1

The Plasmodium falciparum erythrocyte membrane protein 1 (PfEMP1) family plays a crucial role in parasite survival, transmission, and virulence.P. falciparum infections' pathogenicity is associated with the type of P. falciparum erythrocyte membrane protein 1 (PfEMP1) expressed on the surface of infected erythrocytes to anchor these to the vascular lining. This tether these erythrocytes to blood vessels or tissue surfaces, preventing spleen-mediated clearance of the parasite and allowing the infection to build. Switching between PfEMP1 proteins will enable parasites to evade host immunity and modify parasite tropism for different microvascular beds. PfEMP1 proteins are large, complex molecules composed of two to nine Duffy binding-like (DBL) or cysteine-rich interdomain region (CIDR) domains, each of 300–650 amino acids. Based on sequence similarity analysis across the seven known P. falciparum var genomes, CIDR and DBL disciplines can be subclassified into the main subclasses DBLα-ζ and CIDRα-δ, and these can be further divided into 147 subtypes. Even within a domain subclass, sequence diversity is high. This biobrick,BBA_K3582102, is designed to express the CIDRa alpha domain of the PfEMP1 protein of the IT4var 07 strain of P.falciparum in E.coli.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 209
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

References

• 1. https://www.rcsb.org/structure/4v3e
• 2. Lavstsen,Turner,Saguti,Magistrado,S. Rask,S. Jespersen,W. Wang,S. Berger, Baraka,M.

Marquard,Seguin-Orlando,Willerslev,P. Gilbert,Lusingu,G. Theander (2012 June 16).Plasmodium falciparum erythrocyte membrane protein 1 domain cassettes 8 and 13 are associated with severe malaria in children.https://doi.org/10.1073/pnas.1120455109

• 3. Clinton K.Y. Lau, Louise Turner,Jakob S. Jespersen, Edward D. Lowe,Bent Petersen,Christian

W. Wang,Jens E.V. Petersen, John Lusingu, Thor G. Theander, Thomas Lavstsen, and Matthew K. Higgins.Structural Conservation Despite Huge Sequence Diversity Allows EPCR Binding by the PfEMP1 Family Implicated in Severe Childhood Malaria.

• 4. The role of PfEMP1 adhesion domain classification in Plasmodium falciparum pathogenesis

research,Joseph D. Smith .2014 Jul 23.PubMed Central (PMC)https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4159067/