Protein_Domain

Part:BBa_K3582020

Designed by: Avadhoot Jadhav   Group: iGEM20_IISER-Pune-India   (2020-10-19)


Modified C-intein piece

C and N inteins are widely used in the circularizing mechanism of proteins. We plan to use the c-intein to build a composite construction that will help us circularize the protein that we insert within the C and the N inteins. This biobrick is designed by modifying the biobrick BBa_K1362401 designed by the Heidelberg 2014 team(since it was out of the frame and incompatible with RF-cloning). We have modified it to be in frame with the construct making it suitable for performing RF cloning/Gibson's Assembly.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None