Composite
Part:BBa_K358023
Designed by: Ken Kajita Group: iGEM10_Kyoto (2010-10-25)
Lysisbox -type1- ver.2
This part is the same to BBa_K358020[Lysisbox], except for its constitutive promoter[BBa_J23100]. So, this plasmid is able to induce the killer gene more than the plasmid contains BBa_K358020.
This part also contains SΔTMD1, anti-killergene, regulated by lacP.
When transform this part, you must induce the SΔTMD1 highly, do not repress lacP, so that the cell lysis will be prevented.
This is a long part and a lactose operon was deleted sometimes unexpectedly in our experiments. So, we recomend you that it is better to set the cultural temperature at 30C and store this part as a plasmid, not a glycerole stock.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1253
Illegal NheI site found at 1276 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1025
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Categories
Parameters
//function/celldeath
None |