Plasmid

Part:BBa_K3579023:Design

Designed by: Amandine Maire   Group: iGEM20_Paris_Bettencourt   (2020-10-27)


EpiFlex_pPBEF1_mCherry_AB


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3509
    Illegal SpeI site found at 1682
    Illegal PstI site found at 1812
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3509
    Illegal NheI site found at 7740
    Illegal SpeI site found at 1682
    Illegal PstI site found at 1812
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3509
    Illegal BglII site found at 1539
    Illegal BglII site found at 1621
    Illegal BamHI site found at 207
    Illegal BamHI site found at 1667
    Illegal XhoI site found at 1660
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3509
    Illegal SpeI site found at 1682
    Illegal PstI site found at 1812
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3509
    Illegal SpeI site found at 1682
    Illegal PstI site found at 1812
    Illegal NgoMIV site found at 7888
    Illegal AgeI site found at 809
    Illegal AgeI site found at 7357
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 13
    Illegal BsaI site found at 103
    Illegal BsaI site found at 175
    Illegal BsaI site found at 994
    Illegal BsaI site found at 8007
    Illegal BsaI.rc site found at 65
    Illegal BsaI.rc site found at 137
    Illegal BsaI.rc site found at 956
    Illegal BsaI.rc site found at 1105
    Illegal BsaI.rc site found at 7154
    Illegal SapI site found at 4265
    Illegal SapI site found at 5041


Design Notes

BsaI and BbsI restriction sites have been removed



Source

pRMC2 (Plasmid #68940)


References