Regulatory

Part:BBa_K3578000:Design

Designed by: Ruyi Shi   Group: iGEM20_Worldshaper-Nanjing   (2020-07-06)


EXP Promoter


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 756
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 817
    Illegal SpeI site found at 756
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 756
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 756
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

BsaI and SapI restriction enzymes were not present in this sequence.


Source

Promoter from the genome of Yarrowia lipolytica.

References