DNA

Part:BBa_K3570000:Design

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-09-28)


GGPP production enhancement in S. cerevisiae


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 1015
    Illegal PstI site found at 50
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 683
    Illegal NheI site found at 3586
    Illegal NheI site found at 4491
    Illegal NheI site found at 5834
    Illegal PstI site found at 50
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1641
    Illegal BglII site found at 3716
    Illegal BglII site found at 5725
    Illegal BglII site found at 5785
    Illegal BamHI site found at 3376
    Illegal XhoI site found at 705
    Illegal XhoI site found at 3415
    Illegal XhoI site found at 3547
    Illegal XhoI site found at 4366
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 1015
    Illegal PstI site found at 50
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 1015
    Illegal PstI site found at 50
    Illegal NgoMIV site found at 870
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Optimized for S. Cerevisiae


Source

DPP HA up/down - S. Cerevisiae genome HMG1 - S. Cerevisiae genome CrtE - X. Dendrorhous genome Promoter TDH3 - S. Cerevisiae genome Promoter TEF1 - S. Cerevisiae genome Terminator cyc1 - S. Cerevisiae genome Terminator pgk - S. Cerevisiae genome HIS selective locus - S. Cerevisiae genome


References