Part:BBa_K3561028

TSNAVAWTLRCL-CBD

This fusion is a fusion of our palladium reducing peptide and cellulose binding domain. The palladium reducing peptide is expected to reduce palladium and the cellulose binding domain is expected to adsorb to cellulose. More details of the palladium reducing peptide can be found in part BBa_K3561013, this is one of the best performing peptides. The sequence of the cellulose binding domain is provided by Prod. Shen Long Tsai and can be found in part BBa_K3561026.

We have added a NheI and a PStI cut site at the start and end of the sequence respectively. An EcoRI cut site is added in between the peptide and the CBD. The codons are also optimised.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal EcoRI site found at 37
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal EcoRI site found at 37
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal EcoRI site found at 37
  
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal EcoRI site found at 37
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal EcoRI site found at 37
  Illegal AgeI site found at 145
  Illegal AgeI site found at 355
  
• 1000
  COMPATIBLE WITH RFC[1000]

References

Yunus, Ian Sofian, and Shen-Long Tsai. “Designed Biomolecule–Cellulose Complexes for Palladium Recovery and Detoxification.” RSC Advances, The Royal Society of Chemistry, 12 Feb. 2015, pubs.rsc.org/en/content/articlelanding/2015/ra/c4ra16200e.