Generator
CqsA

Part:BBa_K356001:Design

Designed by: C David Owen, James Taylor   Group: iGEM10_St_Andrews   (2010-09-01)


CAI-1 Generator


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 71
    Illegal suffix found in sequence at 1457
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 71
    Illegal NheI site found at 27
    Illegal NheI site found at 50
    Illegal NheI site found at 99
    Illegal NheI site found at 122
    Illegal SpeI site found at 1458
    Illegal PstI site found at 1472
    Illegal NotI site found at 77
    Illegal NotI site found at 1465
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 71
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 71
    Illegal suffix found in sequence at 1458
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 71
    Illegal XbaI site found at 86
    Illegal SpeI site found at 1458
    Illegal PstI site found at 1472
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

CqsA codon optimized by Mr Gene. Biobrick scars were placed between all parts as would be produced by standard assembly.


Source

The CqsA coding reigon (K356000) comes from V. cholerae genomic sequence (NCBI database) synthesized by GeneArt. The rest of the subparts are from the registry.

References