Coding
CqsA

Part:BBa_K356000:Design

Designed by: C David Owen, James Taylor   Group: iGEM10_St_Andrews   (2010-09-01)


CAI-1 autoinducer synthase


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal prefix found in sequence at 8
    Illegal suffix found in sequence at 1394
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 8
    Illegal NheI site found at 36
    Illegal NheI site found at 59
    Illegal SpeI site found at 1395
    Illegal PstI site found at 1409
    Illegal NotI site found at 14
    Illegal NotI site found at 1402
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 8
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal prefix found in sequence at 8
    Illegal suffix found in sequence at 1395
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal prefix found in sequence at 8
    Illegal XbaI site found at 23
    Illegal SpeI site found at 1395
    Illegal PstI site found at 1409
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for expression in E. coli.


Source

Vibrio cholerae genomic sequence, from NCBI database, synthesized by GeneArt.

References