Coding
Part:BBa_K3560001:Design
Designed by: ShiDi Xiao Group: iGEM20_XHD-Wuhan-China (2020-10-21)
gabY
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 138
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 52
Illegal NgoMIV site found at 144
Illegal NgoMIV site found at 310 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
gabY is derived from a pathogenic bacteria, so we cannot clone gabY from the strain. We found the gabY sequence on NCBI, and synthesized the gabY gene fragment by providing the sequence to the biosynthesis company.
Source
We know the sequence of gabY gene, synthesize the gabY gene fragment through a biological company.