Signalling

Part:BBa_K3544009:Design

Designed by: 博宇 邹   Group: iGEM20_SCU-China   (2020-10-22)


NLS: Nuclear localization sequence


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 4
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 4
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 4
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Nuclear localization signal of SV40 large T antigen which can transfer mRNA into nucleus in yeast.

Source

SV40 large T antigen

References

Kitamura, Akira et al. “Efficient and dynamic nuclear localization of green fluorescent protein via RNA binding.” Biochemical and biophysical research communications vol. 463,3 (2015): 401-6. doi:10.1016/j.bbrc.2015.05.084