Intermediate

Part:BBa_K3544008:Design

Designed by: 博宇 邹   Group: iGEM20_SCU-China   (2020-10-22)


CRISPR(Csy4)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

cite for Csy4 cleavage, more information https://2020.igem.org/Team:SCU-China/Description#Eukaryotic

Source

CRISPR sequence, more information https://2020.igem.org/Team:SCU-China/Description#Eukaryotic

References

Qin W, Liang F, Feng Y, et al. Expansion of CRISPR/Cas9 genome targeting sites in zebrafish by Csy4-based RNA processing[J]. Cell research, 2015, 25(9): 1074-1077.